Results

A. Yield of hBcl-2 Transgenic B Cells

B. Survival of hBcl-2 Transgenic B Cells in Culture

C. Surface Phenotype of hBcl-2 Transgenic B Cells

D. Functional Responses in Freshly Isolated hBcl-2 Transgenic and Wild-Type B Cells

E. Effects of Culture on B-Cell Surface Marker Phenotype

F.  Functional Responses in Cultured hBcl-2 Transgenic B Cells

G. Gene Expression Changes in Response to In Vitro Culture and Anti-IgM Stimulation

H. Targeted Manipulation of Signaling Protein Expression